PAXIP1 functions as a key component of the DNA damage response, and phosphoserine or phosphothreonine-specific binding modules. PAXIP1 recognizes substrates phosphorylated by the kinases ataxia telangiectasia-mutated (ATM), ataxia telangiectasia and RAD3-related (ATR) in response to gamma irradiation. It may play a role in PAX2-dependent transcriptional regulation. PAXIP1 plays a role in early development, most probably in cell proliferation.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PAXIP1.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
PAXIP1 antibody can be used for detection of PAXIP1 by ELISA at 1:62500. PAXIP1 antibody can be used for detection of PAXIP1 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human 721_B at 0.2-1 ug/ml.
Positive Control
1) 721_B Cell Lysate
Application Notes
PAXIP1 antibody can be used for detection of PAXIP1 by ELISA at 1:62500. PAXIP1 antibody can be used for detection of PAXIP1 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store PAXIP1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.