PBRM1 is involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology).1) Xia, W., (2008) Cancer Res. 68 (6), 1667-1674.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PBRM1.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
PBRM1 antibody can be used for detection of PBRM1 by ELISA at 1:1562500. PBRM1 antibody can be used for detection of PBRM1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Hum. Fetal Brain at 1 ug/ml.
Application
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.
Positive Control
1) Cat. No. 1211 - HepG2 Cell Lysate
Application Notes
PBRM1 antibody can be used for detection of PBRM1 by ELISA at 1:1562500. PBRM1 antibody can be used for detection of PBRM1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store PBRM1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.