WB
Western blot analysis of BAF180 on different lysates with Rabbit anti-BAF180 antibody at 1/1,000 dilution. Lane 1: HEK-293 cell lysate (20 µg/Lane), Lane 2: HeLa cell lysate (20 µg/Lane), Lane 3: MCF7 cell lysate (20 µg/Lane), Lane 4: A549 cell lysate (20 µg/Lane), Lane 5: SK-MEL-28 cell lysate (20 µg/Lane), Lane 6: Jurkat cell lysate (20 µg/Lane), Lane 7: 293T cell lysate (20 µg/Lane), Lane 8: C6 cell lysate (20 µg/Lane), Lane 9: COS-1 cell lysate (20 µg/Lane), Lane 10: Human kidney tissue lysate (40 µg/Lane), Exposure time: 1 minute 41 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-BAF180 antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-BAF180 antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IF-P
Immunofluorescence analysis of paraffin-embedded human breast tissue labeling BAF180 with Rabbit anti-BAF180 antibody at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).IF-P
Immunofluorescence analysis of paraffin-embedded mouse breast tissue labeling BAF180 with Rabbit anti-BAF180 antibody at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).| Product Name | BAF180 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human BAF180 aa 1-300 / 1,689. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-PIHCWB |
| WB:1:2000 IHC:1:200 IF-P:1:50 |
|
| Species Reactivity | HumanMonkeyMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | PBRM1 |
|---|---|
| Gene Synonyms | PB1 RCC BAF180 SMARCH1 |
| Gene Full Name | polybromo 1 |
| Gene Summary | This locus encodes a subunit of ATP-dependent chromatin-remodeling complexes. The encoded protein has been identified as in integral component of complexes necessary for ligand-dependent transcriptional activation by nuclear hormone receptors. Mutations at this locus have been associated with primary clear cell renal cell carcinoma. [provided by RefSeq, Feb 2012] |
| Alternative Names | BAF180 antibody BRG1-associated factor 180 antibody CG11375 antibody hPB1 antibody MGC156155 antibody MGC156156 antibody OTTHUMP00000202149 antibody OTTHUMP00000202150 antibody OTTHUMP00000202151 antibody OTTHUMP00000202153 antibody BAF180 antibody BRG1-associated factor 180 antibody CG11375 antibody hPB1 antibody MGC156155 antibody MGC156156 antibody OTTHUMP00000202149 antibody OTTHUMP00000202150 antibody OTTHUMP00000202151 antibody OTTHUMP00000202153 antibody OTTHUMP00000202163 antibody PB1 antibody PB1_HUMAN antibody Pbrm1 antibody polybromo 1 antibody Polybromo-1D antibody Protein polybromo-1 antibody |
| Molecular Weight(MW) | 193 kDa |
| Cellular Localization | Nucleus. |
WB
Western blot analysis of BAF180 on different lysates with Rabbit anti-BAF180 antibody at 1/1,000 dilution. Lane 1: HEK-293 cell lysate (20 µg/Lane), Lane 2: HeLa cell lysate (20 µg/Lane), Lane 3: MCF7 cell lysate (20 µg/Lane), Lane 4: A549 cell lysate (20 µg/Lane), Lane 5: SK-MEL-28 cell lysate (20 µg/Lane), Lane 6: Jurkat cell lysate (20 µg/Lane), Lane 7: 293T cell lysate (20 µg/Lane), Lane 8: C6 cell lysate (20 µg/Lane), Lane 9: COS-1 cell lysate (20 µg/Lane), Lane 10: Human kidney tissue lysate (40 µg/Lane), Exposure time: 1 minute 41 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-BAF180 antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-BAF180 antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IF-P
Immunofluorescence analysis of paraffin-embedded human breast tissue labeling BAF180 with Rabbit anti-BAF180 antibody at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
IF-P
Immunofluorescence analysis of paraffin-embedded mouse breast tissue labeling BAF180 with Rabbit anti-BAF180 antibody at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).| Application Notes | WB:1:2000 IHC:1:200 IF-P:1:50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
