PC4 is a transcriptional coactivator. General transcription factor IIH protects promoters from PC4-mediated repression by relieving the topological constraint imposed by PC4 through the ERCC3 helicase activity rather than by reducing the repressive activity of PC4 via its phosphorylation.1) Banerjee, S., et al., (2004) Mol. Cell. Biol. 24 (5), 2052-2062.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PC4.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAIHCWB
PC4 antibody can be used for detection of PC4 by ELISA at 1:1562500. PC4 antibody can be used for detection of PC4 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
PC4 antibody can be used for detection of PC4 by ELISA at 1:1562500. PC4 antibody can be used for detection of PC4 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store PC4 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt