Application
Antibody used in IHC on Human Liver.Application
Antibody used in WB on Human HepG2 at 0.5-1.0 ug/ml.| Product Name | PC4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PC4, P15, PC4, p14 |
| Product description | PC4 (activated RNA polymerase II transcription cofactor 4) is a transcriptional coactivator, possessing the ability to suppress promoter-driven as well as nonspecific transcription via its DNA binding activity. The repressive activity of PC4 on promoter-driven transcription is alleviated by transcription factor TFIIH. TFIIH protects promoters from PC4-mediated repression by relieving the topological constraint imposed by PC4 through the ERCC3 helicase activity.1) Banerjee, S., et al., (2004) Cell.Biol.24(5), 2052-2062. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PC4. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| PC4 antibody can be used for detection of PC4 by ELISA at 1:7812500. PC4 antibody can be used for detection of PC4 by western blot at 0.5-1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | DogHumanMouseRatZebra Fish |
| Concentration | 1mg/ml |
| Gene Symbol | SUB1 |
|---|---|
| Alternative Names | PC4 P15 PC4 p14 |
| Molecular Weight(MW) | 14 kDa |
Application
Antibody used in IHC on Human Liver.
Application
Antibody used in WB on Human HepG2 at 0.5-1.0 ug/ml.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | PC4 antibody can be used for detection of PC4 by ELISA at 1:7812500. PC4 antibody can be used for detection of PC4 by western blot at 0.5-1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store PC4 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 200 μL of distilled water. Final antibody concentration is 1 mg/mL. |
