Application
Western blot analysis in U251 cell line lysates (35ug/lane).| Product Name | PCAF Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Histone acetyltransferase KAT2B, Histone acetyltransferase PCAF, Histone acetylase PCAF, Lysine acetyltransferase 2B, P300/CBP-associated factor, P/CAF, KAT2B, PCAF |
| Product description | CBP and p300 are large nuclear proteins that bind to many sequence-specific factors involved in cell growth and/or differentiation, including c-jun and the adenoviral oncoprotein E1A. The protein encoded by this gene associates with p300/CBP. It has in vitro and in vivo binding activity with CBP and p300, and competes with E1A for binding sites in p300/CBP. It has histone acetyl transferase activity with core histones and nucleosome core particles, indicating that this protein plays a direct role in transcriptional regulation.1) Perez, R.E., et al. J. Cell. Physiol. 225(2):394-405(2010) |
| Immunogen | This PCAF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 802-832 amino acids from the C-terminal region of human PCAF. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | KAT2B |
|---|---|
| Alternative Names | Histone acetyltransferase KAT2B Histone acetyltransferase PCAF Histone acetylase PCAF Lysine acetyltransferase 2B P300/CBP-associated factor P/CAF KAT2B PCAF |
| Molecular Weight(MW) | 93 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Western blot analysis in U251 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
