Application
Western blot analysis of PCCB Antibody in Jurkat cell line lysates (35ug/lane)Application
Flow cytometry analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | PCCB Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Propionyl-CoA carboxylase beta chain, mitochondrial, PCCase subunit beta, Propanoyl-CoA:carbon dioxide ligase subunit beta, PCCB |
| Product description | PCCB is a subunit of the propionyl-CoA carboxylase (PCC) enzyme, which is involved in the catabolism of propionyl-CoA. PCC is a mitochondrial enzyme that probably acts as a dodecamer of six alpha subunits and six beta subunits.1) Yang,X., et.al.,Mol. Genet. Metab. 81 (4), 335-342 (2004) |
| Immunogen | This PCCB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 82-107 amino acids from the Central region of human PCCB. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PCCB |
|---|---|
| Alternative Names | Propionyl-CoA carboxylase beta chain mitochondrial PCCase subunit beta Propanoyl-CoA:carbon dioxide ligase subunit beta PCCB |
| Molecular Weight(MW) | 58 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Western blot analysis of PCCB Antibody in Jurkat cell line lysates (35ug/lane)
Application
Flow cytometry analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
