Application
Confocal immunofluorescent analysis of PCDH1 Antibody with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).Application
PCDH1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western blot analysis in MDA-MB453,ZR-75-1 cell line lysates (35ug/lane).| Product Name | PCDH1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | This gene belongs to the protocadherin subfamily within the cadherin superfamily. The encoded protein is a membrane protein found at cell-cell boundaries. It is involved in neural cell adhesion, suggesting a possible role in neuronal development. The protein includes an extracelllular region, containing 7 cadherin-like domains, a transmembrane region and a C-terminal cytoplasmic region. Cells expressing the protein showed cell aggregation activity. Alternative splicing occurs in this gene.1) Koppelman, G.H., et al. Am. J. Respir. Crit. Care Med. 180(10):929-935(2009) |
| Immunogen | This PCDH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 179-206 amino acids from the N-terminal region of human PCDH1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFIHC-PWB |
| For IHC-P starting dilution is: 1:10~50 For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PCDH1 |
|---|---|
| Alternative Names | Protocadherin-1 Cadherin-like protein 1 Protocadherin-42 PC42 PCDH1 |
| Molecular Weight(MW) | 115 kDa |
Application
Confocal immunofluorescent analysis of PCDH1 Antibody with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Application
PCDH1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western blot analysis in MDA-MB453,ZR-75-1 cell line lysates (35ug/lane).| Application Notes | For IHC-P starting dilution is: 1:10~50 For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
