Application
Immunohistochemistry of PCDH12 in rat lung tissue with PCDH12 antibody at 5 μg/mL.Application
Western blot analysis of PCDH12 in K562 cell lysate with PCDH12 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Product Name | PCDH12 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PCDH12 Antibody: VECAD2, VE-cadherin-2, Protocadherin-12, Vascular cadherin-2, VE-cad-2 |
| Product description | PCDH12 Antibody: Protocadherins comprise the largest group within the cadherin family of calcium-dependent cell-cell adhesion molecules. Protocadherin 12 (PCDH12) was initially identified through PCR screening of mouse heart microvascular endothelial cell RNA; further experiments revealed its mRNA to be strongly expressed in highly vascularized organs such as lung and kidney, in addition to glycogen-rich trophoblasts in the placenta. PCDH12-null mice are viable and fertile, but show reduced placental and embryonic sizes when compared to wild-type mice. Further studies showed significant expression changes in 2, 289 genes, including those involved in tissue morphogenesis, angiogenesis, cell-matrix adhesion and migration, immune response and chromatin remodeling.1) Frank M and Kemler R. Protocadherins. Curr. Opin. Cell Biol.2002; 14:557-62. |
| Immunogen | PCDH12 antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human PCDH12. The immunogen is located within amino acids 30 - 80 of PCDH12. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| PCDH12 antibody can be used for detection of PCDH12 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PCDH12 |
|---|---|
| Alternative Names | PCDH12 Antibody: VECAD2 VE-cadherin-2 Protocadherin-12 Vascular cadherin-2 VE-cad-2 |
| Molecular Weight(MW) | Predicted: 128 kDa Observed: 125 kDa |
| Tissue Specificity | This antibody is predicted to not cross-react with PCDH18. |
Application
Immunohistochemistry of PCDH12 in rat lung tissue with PCDH12 antibody at 5 μg/mL.
Application
Western blot analysis of PCDH12 in K562 cell lysate with PCDH12 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1204 - K562 Cell Lysate |
|---|---|
| Application Notes | PCDH12 antibody can be used for detection of PCDH12 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | PCDH12 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | PCDH12 Antibody is supplied in PBS containing 0.02% sodium azide. |
