WB
SP2/0(Mouse) Cell Lysate at 30 ug, Primary: Anti-PCDH12 at 1/1000 dilution, Secondary: Goat Anti-Rabbit IgG at 1/20000 dilution.FC
Positive control: (mo)Splenocytes(2% Paraformaldehyde-fixed ) Isotype Control Antibody: Rabbit IgG; Dilution: 1μg in 100 μl 1 X PBS containing 0.5% BSA, Secondary Antibody: Goat anti-rabbit IgG-FITC;Dilution: 1:200 in 1 X PBS containing 0.5% BSA, Primary Antibody : Rabbit Anti-CD28 antibody ; Dilution: 1μg in 100 μl 1X PBS containing 0.5% BSA.| Product Name | PCDH12 | VE-cadherin-2 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | KLH conjugated synthetic peptide derived from human PCDH12 : 401-500/1184 . |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCWB |
| WB:1:500-1:2000 FC:1μg/Test |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | PCDH12 |
|---|---|
| Gene Synonyms | DMJDS1 VECAD2 VE-cadherin-2 |
| Gene Full Name | protocadherin 12 |
| Gene Summary | This gene belongs to the protocadherin gene family, a subfamily of the cadherin superfamily. The encoded protein consists of an extracellular domain containing 6 cadherin repeats, a transmembrane domain and a cytoplasmic tail that differs from those of the classical cadherins. The gene localizes to the region on chromosome 5 where the protocadherin gene clusters reside. The exon organization of this transcript is similar to that of the gene cluster transcripts, notably the first large exon, but no significant sequence homology exists. The function of this cellular adhesion protein is undetermined but mouse protocadherin 12 does not bind catenins and appears to have no affect on cell migration or growth. [provided by RefSeq, Jul 2008] |
| Alternative Names | DMJDS1 VE-cadherin-2 VECAD2 PCD12_HUMAN PCDH12 Vascular cadherin-2 Vascular endothelial cadherin-2 (VE-cad-2 | VE-cadherin-2) protocadherin 12 |
| Molecular Weight(MW) | 126 kDa |
| Cellular Localization | Cell membrane. Cell junction. |
WB
SP2/0(Mouse) Cell Lysate at 30 ug, Primary: Anti-PCDH12 at 1/1000 dilution, Secondary: Goat Anti-Rabbit IgG at 1/20000 dilution.
FC
Positive control: (mo)Splenocytes(2% Paraformaldehyde-fixed ) Isotype Control Antibody: Rabbit IgG; Dilution: 1μg in 100 μl 1 X PBS containing 0.5% BSA, Secondary Antibody: Goat anti-rabbit IgG-FITC;Dilution: 1:200 in 1 X PBS containing 0.5% BSA, Primary Antibody : Rabbit Anti-CD28 antibody ; Dilution: 1μg in 100 μl 1X PBS containing 0.5% BSA.| Application Notes | WB:1:500-1:2000 FC:1μg/Test |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
