Application
Flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.Application
Confocal immunofluorescent analysis of PCDHAC2 Antibody with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).Application
Western blot analysis in NCI-H460 cell line lysates (35ug/lane).Application
PCDHAC2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Product Name | PCDHAC2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Protocadherin alpha-C2, PCDH-alpha-C2, PCDHAC2 |
| Product description | This gene is a member of the protocadherin alpha gene cluster, one of three related gene clusters tandemly linked on chromosome five that demonstrate an unusual genomic organization similar to that of B-cell and T-cell receptor gene clusters. The alpha gene cluster is composed of 15 cadherin superfamily genes related to the mouse CNR genes and consists of 13 highly similar and 2 more distantly related coding sequences. The tandem array of 15 N-terminal exons, or variable exons, are followed by downstream C-terminal exons, or constant exons, which are shared by all genes in the cluster. The large, uninterrupted N-terminal exons each encode six cadherin ectodomains while the C-terminal exons encode the cytoplasmic domain. These neural cadherin-like cell adhesion proteins are integral plasma membrane proteins that most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been observed and additional variants have been suggested but their full-length nature has yet to be determined.1) Wu, Q., et al. Genome Res. 11(3):389-404(2001) |
| Immunogen | This PCDHAC2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 616-644 amino acids from the Central region of human PCDHAC2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIFIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PCDHAC2 |
|---|---|
| Alternative Names | Protocadherin alpha-C2 PCDH-alpha-C2 PCDHAC2 |
| Molecular Weight(MW) | 109 kDa |
Application
Flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
Application
Confocal immunofluorescent analysis of PCDHAC2 Antibody with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
Application
Western blot analysis in NCI-H460 cell line lysates (35ug/lane).
Application
PCDHAC2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
