Application
Western blot analysis in NCI-H292 cell line lysates (35ug/lane).Application
PCDHB12 Antibdy immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PCDHB12 Antibdy for immunohistochemistry.| Product Name | PCDHB12 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Protocadherin beta-12, PCDH-beta-12, PCDHB12 |
| Product description | This gene is a member of the protocadherin beta gene cluster, one of three related gene clusters tandemly linked on chromosome five. The gene clusters demonstrate an unusual genomic organization similar to that of B-cell and T-cell receptor gene clusters. The beta cluster contains 16 genes and 3 pseudogenes, each encoding 6 extracellular cadherin domains and a cytoplasmic tail that deviates from others in the cadherin superfamily. The extracellular domains interact in a homophilic manner to specify differential cell-cell connections. Unlike the alpha and gamma clusters, the transcripts from these genes are made up of only one large exon, not sharing common 3' exons as expected. These neural cadherin-like cell adhesion proteins are integral plasma membrane proteins. Their specific functions are unknown but they most likely play a critical role in the establishment and function of specific cell-cell neural connections.1) Vanhalst, K., et al. FEBS Lett. 495 (1-2), 120-125 (2001) : |
| Immunogen | This PCDHB12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 766-795 amino acids from the C-terminal region of human PCDHB12. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PCDHB12 |
|---|---|
| Alternative Names | Protocadherin beta-12 PCDH-beta-12 PCDHB12 |
| Molecular Weight(MW) | 87 kDa |
Application
Western blot analysis in NCI-H292 cell line lysates (35ug/lane).
Application
PCDHB12 Antibdy immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PCDHB12 Antibdy for immunohistochemistry.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
