Application
Antibody used in WB on Hum. Adult Placenta at 1 ug/ml.Application
Antibody used in WB on Human 293T at 1 ug/ml.Application
Antibody used in WB on Human 721_B at 0.2-1 ug/ml.| Product Name | PCDHGC3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | This gene is a member of the protocadherin gamma gene cluster, one of three related clusters tandemly linked on chromosome five. These gene clusters have an immunoglobulin-like organization, suggesting that a novel mechanism may be involved in their regulation and expression. PCDHGC3 is a potential calcium-dependent cell-adhesion protein. It may be involved in the establishment and maintenance of specific neuronal connections in the brain.This gene is a member of the protocadherin gamma gene cluster, one of three related clusters tandemly linked on chromosome five. These gene clusters have an immunoglobulin-like organization, suggesting that a novel mechanism may be involved in their regulation and expression. The gamma gene cluster includes 22 genes divided into 3 subfamilies. Subfamily A contains 12 genes, subfamily B contains 7 genes and 2 pseudogenes, and the more distantly related subfamily C contains 3 genes. The tandem array of 22 large, variable region exons are followed by a constant region, containing 3 exons shared by all genes in the cluster. Each variable region exon encodes the extracellular region, which includes 6 cadherin ectodomains and a transmembrane region. The constant region exons encode the common cytoplasmic region. These neural cadherin-like cell adhesion proteins most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been described for the gamma cluster genes.1) Reiss, K., (2006) J. Biol. Chem. 281 (31), 21735-21744. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PCDHGC3. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| PCDHGC3 antibody can be used for detection of PCDHGC3 by ELISA at 1:312500. PCDHGC3 antibody can be used for detection of PCDHGC3 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PCDHGC3 |
|---|---|
| Alternative Names | PCDHGC3 PC43 PCDH-GAMMA-C3 PCDH2 |
| Molecular Weight(MW) | 98 kDa |
Application
Antibody used in WB on Hum. Adult Placenta at 1 ug/ml.
Application
Antibody used in WB on Human 293T at 1 ug/ml.
Application
Antibody used in WB on Human 721_B at 0.2-1 ug/ml.| Positive Control | 1) 721_B Cell Lysate |
|---|---|
| Application Notes | PCDHGC3 antibody can be used for detection of PCDHGC3 by ELISA at 1:312500. PCDHGC3 antibody can be used for detection of PCDHGC3 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store PCDHGC3 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
