Application
Confocal immunofluorescent analysis of PCK2 Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).Application
Western blot analysis of PCK2 using rabbit polyclonal PCK2 Antibody (Q39) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PCK2 gene.Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.Application
Western blot analysis of anti-PCK2 Antibody in HepG2 cell line lysates (35ug/lane)| Product Name | PCK2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Phosphoenolpyruvate carboxykinase [GTP], mitochondrial, PEPCK-M, PCK2, PEPCK2 |
| Product description | This gene encodes a member of the phosphoenolpyruvate carboxykinase (GTP) family. The protein is a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of GTP. A cytosolic form encoded by a different gene has also been characterized and is the key enzyme of gluconeogenesis in the liver. The encoded protein may serve a similar function, although it is constitutively expressed and not modulated by hormones such as glucagon and insulin that regulate the cytosolic form. Alternatively spliced transcript variants have been described.1) Strausberg, R.L., et al., Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903 (2002). |
| Immunogen | This PCK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 24-54 amino acids from the N-terminal region of human PCK2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For IF starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PCK2 |
|---|---|
| Alternative Names | Phosphoenolpyruvate carboxykinase [GTP] mitochondrial PEPCK-M PCK2 PEPCK2 |
| Molecular Weight(MW) | 71 kDa |
Application
Confocal immunofluorescent analysis of PCK2 Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Application
Western blot analysis of PCK2 using rabbit polyclonal PCK2 Antibody (Q39) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PCK2 gene.
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.
Application
Western blot analysis of anti-PCK2 Antibody in HepG2 cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For IF starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
