Application
Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
PCOTH antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western blot analysis in 293 cell line lysates (35ug/lane).| Product Name | PCOTH Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Prostate collagen triple helix protein, C1QTNF9B antisense RNA 1, C1QTNF9B antisense gene protein 1, C1QTNF9B-AS1, PCOTH |
| Product description | May be involved in growth and survival of prostate cancer cells through the TAF-Ibeta pathway.1) Anazawa, Y., et al. Cancer Res. 65(11):4578-4586(2005) |
| Immunogen | This PCOTH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 14-42 amino acids from the Central region of human PCOTH. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | C1QTNF9B-AS1 |
|---|---|
| Alternative Names | Prostate collagen triple helix protein C1QTNF9B antisense RNA 1 C1QTNF9B antisense gene protein 1 C1QTNF9B-AS1 PCOTH |
| Molecular Weight(MW) | 11 kDa |
Application
Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
PCOTH antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western blot analysis in 293 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
