Application
Confocal immunofluorescent analysis of PCSK2 Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).Application
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western Blot at 1:1000 dilution + TT whole cell lysate Lysates/proteins at 20 ug per lane.Application
Western blot analysis of PCSK2 Antibody in mouse cerebellum tissue lysates (35ug/lane)Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with PCSK2 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Product Name | PCSK2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Neuroendocrine convertase 2, NEC 2, KEX2-like endoprotease 2, Prohormone convertase 2, Proprotein convertase 2, PC2, PCSK2, NEC2 |
| Product description | PCSK2 belongs to the subtilisin-like proprotein convertase family. The members of this family are proprotein convertases that process latent precursor proteins into their biologically active products. This protein is a proinsulin-processing enzyme that plays a key role in regulating insulin biosynthesis. The protein is also known to cleave proopiomelanocortin, proenkephalin, prodynorphin and proluteinizing-hormone-releasing hormone.1) Leak,T.S., Keene,K.L. Mol. Genet. Metab. 92 (1-2), 145-150 (2007) |
| Immunogen | This PCSK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 87-116 amino acids from the N-terminal region of human PCSK2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIFIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Gene Symbol | PCSK2 |
|---|---|
| Alternative Names | Neuroendocrine convertase 2 NEC 2 KEX2-like endoprotease 2 Prohormone convertase 2 Proprotein convertase 2 PC2 PCSK2 NEC2 |
| Molecular Weight(MW) | 71 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Pig |
Application
Confocal immunofluorescent analysis of PCSK2 Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Application
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western Blot at 1:1000 dilution + TT whole cell lysate Lysates/proteins at 20 ug per lane.
Application
Western blot analysis of PCSK2 Antibody in mouse cerebellum tissue lysates (35ug/lane)
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with PCSK2 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
