Application
Confocal immunofluorescent analysis of PCYT1A Antibody with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).Application
PCYT1A Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western Blot at 1:1000 dilution Lane 1: HT-1080 whole cell lysate Lane 2: K562 whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | PCYT1A Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Choline-phosphate cytidylyltransferase A, CCT-alpha, CTP:phosphocholine cytidylyltransferase A, CCT A, CT A, Phosphorylcholine transferase A, PCYT1A, CTPCT, PCYT1 |
| Product description | PCYT1A controls phosphatidylcholine synthesis.1) Mostowska, A., et al. J. Med. Genet. (2010) In press : |
| Immunogen | This PCYT1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 27-54 amino acids from the N-terminal region of human PCYT1A. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PCYT1A |
|---|---|
| Alternative Names | Choline-phosphate cytidylyltransferase A CCT-alpha CTP:phosphocholine cytidylyltransferase A CCT A CT A Phosphorylcholine transferase A PCYT1A CTPCT PCYT1 |
| Molecular Weight(MW) | 42 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Confocal immunofluorescent analysis of PCYT1A Antibody with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Application
PCYT1A Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western Blot at 1:1000 dilution Lane 1: HT-1080 whole cell lysate Lane 2: K562 whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
