Application
Immunohistochemistry of PD-1 in human brain tissue with PD-1 antibody at 5 μg/mL.Application
Western blot analysis of PD-1 in THP-1 cell lysate with PD-1 antibody at 1 μg/mL in the (A) absence and (B) presence of blocking peptide.WB
(e) The representative western blot bands of PD-1 and PD-L1 around lesion area.IF
(h and i) PD1 and PD-L1 cells around the lesion area were labeled by immunofluorescence (n = 3, each sample was chosen two fields of view).IF
(h and i) PD1 and PD-L1 cells around the lesion area were labeled by immunofluorescence (n = 3, each sample was chosen two fields of view).| Product Name | PD-1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | PD-1 Antibody: Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antig en-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of the immune response in the absence of "danger signals" such as LPS or other molecules associated with bacteria or other pathogens. Evidence for this is seen in PD1-null mice who exhibit hyperactivated immune systems and autoimmune diseases.1) Holling TM, Schooten E, and van Den Elsing PJ. Function and regulation of MHC class II molecules in T-lymphocytes: of mice and men. Hum. Immunol. 2004; 65:282-90. |
| Immunogen | PD-1 antibody was raised against a 16 amino acid synthetic peptide from near the center of human PD-1. The immunogen is located within amino acids 120 - 170 of PD-1. |
| Modification | Acetylated |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| PD-1 antibody can be used for detection of PD-1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PDCD1 |
|---|---|
| Alternative Names | PD-1 Antibody: PD1 PD-1 CD279 SLEB2 hPD-1 hPD-l hSLE1 PD1 Programmed cell death protein 1 Protein PD-1 |
| Molecular Weight(MW) | Predicted: 32 kDa Observed: 43 kDa |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
Application
Immunohistochemistry of PD-1 in human brain tissue with PD-1 antibody at 5 μg/mL.
Application
Western blot analysis of PD-1 in THP-1 cell lysate with PD-1 antibody at 1 μg/mL in the (A) absence and (B) presence of blocking peptide.
WB
(e) The representative western blot bands of PD-1 and PD-L1 around lesion area.
IF
(h and i) PD1 and PD-L1 cells around the lesion area were labeled by immunofluorescence (n = 3, each sample was chosen two fields of view).
IF
(h and i) PD1 and PD-L1 cells around the lesion area were labeled by immunofluorescence (n = 3, each sample was chosen two fields of view).| Positive Control | 1) Cat. No. 1208 - THP-1 Cell Lysate |
|---|---|
| Application Notes | PD-1 antibody can be used for detection of PD-1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | PD-1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | PD-1 Antibody is supplied in PBS containing 0.02% sodium azide. |
