Application
Antibody is used in Western blot to detect PDAP1 in HL60 lysate.Application
Western blot analysis of PDAP1 using rabbit polyclonal PDAP1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PDAP1 gene (Lane 2).| Product Name | PDAP1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 28 kDa heat- and acid-stable phosphoprotein, PDGF-associated protein, PAP, PDGFA-associated protein 1, PAP1, PDAP1, HASPP28 |
| Product description | Human PDAP1 encodes a deduced 181-amino acid protein expressed in all tissues test with the exception of liver, most abundantly in brain. PDAP1 enhances PDGFA-stimulated cell growth in mouse fibroblasts, but inhibits the mitogenic effect of PDGFB. The binding between PDGFA and PDAP1 is a low affinity/high capacity interaction, as determined by sold-phase studies.1) Fischer, W.H., et al., J. Neurochem. 66(5):2213-2216 (1996). |
| Immunogen | This PDAP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 142-171 amino acids from the C-terminal region of human PDAP1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | PDAP1 |
|---|---|
| Alternative Names | 28 kDa heat- and acid-stable phosphoprotein PDGF-associated protein PAP PDGFA-associated protein 1 PAP1 PDAP1 HASPP28 |
| Molecular Weight(MW) | 21 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Antibody is used in Western blot to detect PDAP1 in HL60 lysate.
Application
Western blot analysis of PDAP1 using rabbit polyclonal PDAP1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PDAP1 gene (Lane 2).| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
