Application
Immunohistochemistry of PDCL3 in mouse lver tissue with PDCL3 antibody at 5 μg/mL.Application
Western blot analysis of PDCL3 in human brain tissue lysate with PDCL3 antibody at 1 μg/ml.Application
Immunofluorescence of PDCL3 in mouse liver tissue with PDCL3 antibody at 20 μg/mL.| Product Name | PDCL3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PDCL3 Antibody: VIAF, PHLP3, VIAF1, HTPHLP, PHLP2A, PhLP2A, Phosducin-like protein 3, VIAF-1 |
| Product description | Phosducin-like proteins (PhLPs) are a conserved family of proteins with thioredoxin-like domains that were initially identified as modulators of G protein signaling (1,2). PDCL3 is highly homologous to PDCL and shares an N-terminal helix domain and a C-terminal thioredoxin-fold (Trx-fold) domain (3). Along with the related protein PDCL2, PDCL3 interacts with the chaperonin CCT and modulates CCT-mediated actin and tubulin folding (4). Modulation of PDCL3 levels by MAPK phosphorylation and RhoA-dependent changes also promote cytoskeletal remodeling (5).1) Miles MF, Barhite S, Sganga M, et al. Phosducin-like protein: an ethanol-responsive potential modulator of guanine nucleotide-binding protein function. Proc. Natl. Acad. Sci. USA 1993; 90:10831-5. |
| Immunogen | PDCL3 antibody was raised against a 14 amino acid peptide near the carboxy terminus of human PDCL3. The immunogen is located within the last 50 amino acids of PDCL3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| PDCL3 antibody can be used for detection of PDCL by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PDCL3 |
|---|---|
| Alternative Names | PDCL3 Antibody: VIAF PHLP3 VIAF1 HTPHLP PHLP2A PhLP2A Phosducin-like protein 3 VIAF-1 |
| Molecular Weight(MW) | Predicted: 26 kDa Observed: 25 kDa |
| Tissue Specificity | PDCL3 antibody is human, mouse and rat reactive. PDCL3 antibody is predicted to not cross-react with other members of the PDCL protein family. |
Application
Immunohistochemistry of PDCL3 in mouse lver tissue with PDCL3 antibody at 5 μg/mL.
Application
Western blot analysis of PDCL3 in human brain tissue lysate with PDCL3 antibody at 1 μg/ml.
Application
Immunofluorescence of PDCL3 in mouse liver tissue with PDCL3 antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1303 - Human Brain Tissue Lysate |
|---|---|
| Application Notes | PDCL3 antibody can be used for detection of PDCL by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | PDCL3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | PDCL3 antibody is supplied in PBS containing 0.02% sodium azide. |
