Application
Antibody used in WB on Human OVCAR-3 at 0.2-1 ug/ml.| Product Name | PDE Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 2'-PDE, 2'-PDE |
| Product description | 2'-PDE is an enzyme that cleaves 2',5'-phosphodiester bond linking adenosines of the 5'-triphosphorylated oligoadenylates, triphosphorylated oligoadenylates referred as 2-5A modulates the 2-5A system. This enzyme degraded triphosphorylated 2-5A to produce AMP and ATP. 2'-PDE also cleaves 3',5'-phosphodiester bond of oligoadenylates. 2'-PDE play a role as a negative regulator of the 2-5A system that is one of the major pathways for antiviral and antitumor functions induced by interferons (IFNs). Suppression of this enzyme induces reduction of viral replication in Hela cells, thus counteracting the antiviral pathway probably by inhibiting the 2-5A system.1) Kubota, K., (2004) J. Biol. Chem. 279 (36), 37832-37841 . |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PDE. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| PDE antibody can be used for detection of PDE by ELISA at 1:1562500. PDE antibody can be used for detection of PDE by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PDE12 |
|---|---|
| Alternative Names | 2'-PDE 2'-PDE |
| Molecular Weight(MW) | 67 kDa |
Application
Antibody used in WB on Human OVCAR-3 at 0.2-1 ug/ml.| Positive Control | 1) OVCAR-3 Cell Lysate |
|---|---|
| Application Notes | PDE antibody can be used for detection of PDE by ELISA at 1:1562500. PDE antibody can be used for detection of PDE by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store PDE antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
