Application
Western blot analysis in MDA-MB231 cell line lysates (35ug/lane).Application
Flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | PDE12 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 2',5'-phosphodiesterase 12, 2'-PDE, 2-PDE, 314-, Mitochondrial deadenylase, PDE12 |
| Product description | 1) Kohno, T., et al. Acta Crystallogr. Sect.Struct. Biol. Cryst. Commun. 66 (PT 5), 520-522 (2010) : |
| Immunogen | This PDE12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 362-390 amino acids from the C-terminal region of human PDE12. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PDE12 |
|---|---|
| Alternative Names | 2' 5'-phosphodiesterase 12 2'-PDE 2-PDE 314- Mitochondrial deadenylase PDE12 |
| Molecular Weight(MW) | 67 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Western blot analysis in MDA-MB231 cell line lysates (35ug/lane).
Application
Flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
