Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with PDGFA antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Antibody is used in Western blot to detect PDGFA in HL60 cell lysate.| Product Name | PDGFA Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Platelet-derived growth factor subunit A, PDGF subunit A, PDGF-1, Platelet-derived growth factor A chain, Platelet-derived growth factor alpha polypeptide, PDGFA, PDGF1 |
| Product description | PDGFA is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer or as a heterodimer with the platelet-derived growth factor beta polypeptide, where the dimers are connected by disulfide bonds. Studies using knockout mice have shown cellular defects in oligodendrocytes, alveolar smooth muscle cells, and Leydig cells in the testis; knockout mice die either as embryos or shortly after birth.1) Monje, P., et al., Mol. Cell. Biol. 23(19):7030-7043 (2003). Gianni, D., et al., J. Biol. Chem. 278(11):9290-9297 (2003). Muller, C., et al., J. Biol. Chem. 278(20):18330-18335 (2003). Chui, C.M., et al., Cytokine 21(2):51-64 (2003). Laprise, M.H., et al., Blood 100(10):3578-3587 (2002). |
| Immunogen | This PDGFA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 60-89 amino acids from the N-terminal region of human PDGFA. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | PDGFA |
|---|---|
| Alternative Names | Platelet-derived growth factor subunit A PDGF subunit A PDGF-1 Platelet-derived growth factor A chain Platelet-derived growth factor alpha polypeptide PDGFA PDGF1 |
| Molecular Weight(MW) | 24 kDa |
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with PDGFA antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Antibody is used in Western blot to detect PDGFA in HL60 cell lysate.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
