Application
Flow cytometric analysis of U251 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.Application
Western blot analysis of PDGFRA antibody (C-term E1063) in mouse lung tissue lysates (35ug/lane)Application
Western blot analysis of PDGFRA Antibody (C-term E1063) in K562 cell line lysates (35ug/lane)| Product Name | PDGFRA Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Platelet-derived growth factor receptor alpha, PDGF-R-alpha, PDGFR-alpha, Alpha platelet-derived growth factor receptor, Alpha-type platelet-derived growth factor receptor, CD140 antigen-like family member A, CD140a antigen, Platelet-derived growth factor |
| Product description | PDGFRA is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. Studies in knockout mice, where homozygosity is lethal, indicate that the alpha form of the platelet-derived growth factor receptor is particularly important for kidney development since mice heterozygous for the receptor exhibit defective kidney phenotypes.1) Cools, J., et al., N. Engl. J. Med. 348(13):1201-1214 (2003). |
| Immunogen | This PDGFRA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1048-1077 amino acids from the C-terminal region of human PDGFRA. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Gene Symbol | PDGFRA |
|---|---|
| Alternative Names | Platelet-derived growth factor receptor alpha PDGF-R-alpha PDGFR-alpha Alpha platelet-derived growth factor receptor Alpha-type platelet-derived growth factor receptor CD140 antigen-like family member A CD140a antigen Platelet-derived growth factor |
| Molecular Weight(MW) | 123 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Chicken |
Application
Flow cytometric analysis of U251 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.
Application
Western blot analysis of PDGFRA antibody (C-term E1063) in mouse lung tissue lysates (35ug/lane)
Application
Western blot analysis of PDGFRA Antibody (C-term E1063) in K562 cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
