Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PDGFRA Antibody (Y720) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis of PDGFRA using rabbit polyclonal PDGFRA Antibody (Y720) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PDGFRA gene (Lane 2).Application
Western Blot at 1:1000 dilution + human platelet lysates Lysates/proteins at 20 ug per lane.Application
Antibody staining PDGFRA in Human hepatic carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).| Product Name | PDGFRA Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Platelet-derived growth factor receptor alpha, PDGF-R-alpha, PDGFR-alpha, Alpha platelet-derived growth factor receptor, Alpha-type platelet-derived growth factor receptor, CD140 antigen-like family member A, CD140a antigen, Platelet-derived growth factor |
| Product description | PDGFRA is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. Studies in knockout mice, where homozygosity is lethal, indicate that the alpha form of the platelet-derived growth factor receptor is particularly important for kidney development since mice heterozygous for the receptor exhibit defective kidney phenotypes.1) Wilczynski, S.P., et al., Hum. Pathol. 36(3):242-249 (2005). |
| Immunogen | This PDGFRA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 698-727 amino acids from human PDGFRA. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For IHC-P starting dilution is: 1:25 For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PDGFRA |
|---|---|
| Alternative Names | Platelet-derived growth factor receptor alpha PDGF-R-alpha PDGFR-alpha Alpha platelet-derived growth factor receptor Alpha-type platelet-derived growth factor receptor CD140 antigen-like family member A CD140a antigen Platelet-derived growth factor |
| Molecular Weight(MW) | 123 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PDGFRA Antibody (Y720) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis of PDGFRA using rabbit polyclonal PDGFRA Antibody (Y720) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PDGFRA gene (Lane 2).
Application
Western Blot at 1:1000 dilution + human platelet lysates Lysates/proteins at 20 ug per lane.
Application
Antibody staining PDGFRA in Human hepatic carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).| Application Notes | For IHC-P starting dilution is: 1:25 For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
