Application
PDHA2 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western blot analysis in NCI-H460 cell line lysates (35ug/lane).| Product Name | PDHA2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Pyruvate dehydrogenase E1 component subunit alpha, testis-specific form, mitochondrial, PDHE1-A type II, PDHA2, PDHAL |
| Product description | The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).1) Pinheiro, A., et al. Mol. Genet. Metab. 99(4):425-430(2010) |
| Immunogen | This PDHA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 287-314 amino acids from the Central region of human PDHA2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PDHA2 |
|---|---|
| Alternative Names | Pyruvate dehydrogenase E1 component subunit alpha testis-specific form mitochondrial PDHE1-A type II PDHA2 PDHAL |
| Molecular Weight(MW) | 43 kDa |
Application
PDHA2 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western blot analysis in NCI-H460 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
