Application
Immunohistochemistry of PDIA1 in rat small intestine tissue with PDIA1 antibody at 5 μg/mL.Application
Immunofluorescence of PDIA1 in rat small intestine tissue with PDIA1 antibody at 20 μg/mL.Application
Western blot analysis of PDIA1 in rat small intestine tissue lysate with PDIA1 antibody at (A) 1 and (B) 2 μg/ml.| Product Name | PDIA1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Protein disulfide isomerase family A member 1, DSI, GIT, PDI, PHDB, PO4DB, PO4HB, PROHB, ERBA2L, P4Hbeta |
| Product description | PDIA1 (protein disulfide isomerase family A member 1) is the beta subunit of prolyl 4-hydroxylase, a highly abundant multifunctional enzyme that belongs to the protein disulfide isomerase family. When present as a tetramer consisting of two alpha and two beta subunits, this enzyme is involved in hydroxylation of prolyl residues in preprocollagen (1,2). PDIA1 is also a disulfide isomerase containing two thioredoxin domains that catalyze the formation, breakage and rearrangement of disulfide bonds (3). Other known functions include its ability to act as a chaperone that inhibits aggregation of misfolded proteins in a concentration-dependent manner, its ability to bind thyroid hormone, its role in both the influx and efflux of S-nitrosothiol-bound nitric oxide, and its function as a subunit of the microsomal triglyceride transfer protein complex (4).1) Pihlajaniemi T, Helaakoski T, Tasanen K, et al. Molecular cloning of the beta-subunit of human prolyl 4-hydroxylase. This subunit and protein disulphide isomerase are products of the same gene. EMBO J. 1987; 6L:643-9. |
| Immunogen | PDIA1 antibody was raised against a 17 amino acid peptide near the center of human PDIA1. The immunogen is located within amino acids 190 - 240 of PDIA1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| PDIA1 antibody can be used for detection of PDIA1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | P4HB |
|---|---|
| Alternative Names | Protein disulfide isomerase family A member 1 DSI GIT PDI PHDB PO4DB PO4HB PROHB ERBA2L P4Hbeta |
| Molecular Weight(MW) | Predicted: 54 kDa Observed: 54 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Rabbit: (100%), Bovine: (100%), Chicken: (88%) |
| Tissue Specificity | PDIA1 antibody is human, mouse and rat reactive. |
Application
Immunohistochemistry of PDIA1 in rat small intestine tissue with PDIA1 antibody at 5 μg/mL.
Application
Immunofluorescence of PDIA1 in rat small intestine tissue with PDIA1 antibody at 20 μg/mL.
Application
Western blot analysis of PDIA1 in rat small intestine tissue lysate with PDIA1 antibody at (A) 1 and (B) 2 μg/ml.| Positive Control | 1) Cat. No. 1468 - Rat Small Intestine Tissue Lysate |
|---|---|
| Application Notes | PDIA1 antibody can be used for detection of PDIA1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | PDIA1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | PDIA1 antibody is supplied in PBS containing 0.02% sodium azide. |
