PDK2 inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.1) Hiromasa, Y., (2008) Biochemistry 47 (8), 2312-2324.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PDK2.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
PDK2 antibody can be used for detection of PDK2 by ELISA at 1:12500. PDK2 antibody can be used for detection of PDK2 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
PDK2 antibody can be used for detection of PDK2 by ELISA at 1:12500. PDK2 antibody can be used for detection of PDK2 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store PDK2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.