Application
PDK2 Antibody IHC analysis in formalin fixed and paraffin embedded skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Flow cytometric analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis in RD cell line and mouse heart,rat heart lysates (35ug/lane).| Product Name | PDK2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 2, mitochondrial, Pyruvate dehydrogenase kinase isoform 2, PDH kinase 2, PDKII, PDK2, PDHK2 |
| Product description | PDK2 inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.1) Li, J., et al. J. Biol. Chem. 284(49):34458-34467(2009) Fencl, F., et al. Pediatr. Nephrol. 24(5):983-989(2009) Sun, W., et al. Clin. Cancer Res. 15(2):476-484(2009) Hiromasa, Y., et al. Biochemistry 47(8):2312-2324(2008) |
| Immunogen | This PDK2 antibody is generated from rabbits immunized with recombinant protein of human PDK2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PDK2 |
|---|---|
| Alternative Names | [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 2 mitochondrial Pyruvate dehydrogenase kinase isoform 2 PDH kinase 2 PDKII PDK2 PDHK2 |
| Molecular Weight(MW) | 46 kDa |
Application
PDK2 Antibody IHC analysis in formalin fixed and paraffin embedded skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Flow cytometric analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis in RD cell line and mouse heart,rat heart lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
