Application
Antibody used in WB on Transfected 293T at 0.2-1 ug/ml.| Product Name | PDK3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PDK3, CMTX6, GS1-358P8.4 |
| Product description | PDK3 belongs to the PDK/BCKDK protein kinase family. It contains 1 histidine kinase domain. PDK3 inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.1) Degenhardt, T., (2007) J. Mol. Biol. 372 (2), 341-355. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PDK3. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| PDK3 antibody can be used for detection of PDK3 by ELISA at 1:312500. PDK3 antibody can be used for detection of PDK3 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PDK3 |
|---|---|
| Alternative Names | PDK3 CMTX6 GS1-358P8.4 |
| Molecular Weight(MW) | 47 kDa |
Application
Antibody used in WB on Transfected 293T at 0.2-1 ug/ml.| Positive Control | 1) Tranfected 293T Cell Lysate |
|---|---|
| Application Notes | PDK3 antibody can be used for detection of PDK3 by ELISA at 1:312500. PDK3 antibody can be used for detection of PDK3 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store PDK3 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
