Application
PDK3 Antibody FC analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of PDK3 Antibody in MCF-7 cell line lysates (35ug/lane)| Product Name | PDK3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 3, mitochondrial, Pyruvate dehydrogenase kinase isoform 3, PDK3, PDHK3 |
| Product description | PDK3 inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.1) Baker, J.C., et al., J. Biol. Chem. 275(21):15773-15781 (2000). |
| Immunogen | This PDK3 antibody is generated from rabbits immunized with human PDK3 recombinant protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PDK3 |
|---|---|
| Alternative Names | [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 3 mitochondrial Pyruvate dehydrogenase kinase isoform 3 PDK3 PDHK3 |
| Molecular Weight(MW) | 47 kDa |
Application
PDK3 Antibody FC analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of PDK3 Antibody in MCF-7 cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
