Application
Western blot analysis of PDK4 Antibody (E265) in CEM cell line lysates (35ug/lane)Application
Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with PDK4-E265, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Product Name | PDK4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 4, mitochondrial, Pyruvate dehydrogenase kinase isoform 4, PDK4, PDHK4 |
| Product description | PDK4 inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.1) Rosa, G., et al., Obes. Res. 11(2):176-182 (2003). Razeghi, P., et al., Cardiology 97(4):203-209 (2002). Rowles, J., et al., J. Biol. Chem. 271(37):22376-22382 (1996). Gudi, R., et al., J. Biol. Chem. 270(48):28989-28994 (1995). |
| Immunogen | This PDK4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 250-277 amino acids from human PDK4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PDK4 |
|---|---|
| Alternative Names | [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 4 mitochondrial Pyruvate dehydrogenase kinase isoform 4 PDK4 PDHK4 |
| Molecular Weight(MW) | 46 kDa |
Application
Western blot analysis of PDK4 Antibody (E265) in CEM cell line lysates (35ug/lane)
Application
Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with PDK4-E265, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
