Application
Western blot analysis in Jurkat,HepG2,293 cell line lysates (35ug/lane).Application
PDP1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western Blot at 1:2000 dilution Lane 1: 293T/17 whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: L6 whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | PDP1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | [Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 1, mitochondrial, PDP 1, Protein phosphatase 2C, Pyruvate dehydrogenase phosphatase catalytic subunit 1, PDPC 1, PDP1, PDP, PPM2C |
| Product description | Pyruvate dehydrogenase (E1) is one of the three components (E1, E2, and E3) of the large pyruvate dehydrogenase complex. Pyruvate dehydrogenase kinases catalyze phosphorylation of serine residues of E1 to inactivate the E1 component and inhibit the complex. Pyruvate dehydrogenase phosphatases catalyze the dephosphorylation and activation of the E1 component to reverse the effects of pyruvate dehydrogenase kinases. Pyruvate dehydrogenase phosphatase is a heterodimer consisting of catalytic and regulatory subunits. Two catalytic subunits have been reported; one is predominantly expressed in skeletal muscle and another one is is much more abundant in the liver. The catalytic subunit, encoded by this gene, is the former, and belongs to the protein phosphatase 2C (PP2C) superfamily. Along with the pyruvate dehydrogenase complex and pyruvate dehydrogenase kinases, this enzyme is located in the mitochondrial matrix. Mutation in this gene causes pyruvate dehydrogenase phosphatase deficiency. Multiple alternatively spliced transcript variants encoding different isoforms have been identified.1) Kato, J., et al. Acta Crystallogr. Sect.Struct. Biol. Cryst. Commun. 66 (PT 3), 342-345 (2010) : |
| Immunogen | This PDP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 308-336 amino acids from the Central region of human PDP1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:2000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PDP1 |
|---|---|
| Alternative Names | [Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 1 mitochondrial PDP 1 Protein phosphatase 2C Pyruvate dehydrogenase phosphatase catalytic subunit 1 PDPC 1 PDP1 PDP PPM2C |
| Molecular Weight(MW) | 61 kDa |
Application
Western blot analysis in Jurkat,HepG2,293 cell line lysates (35ug/lane).
Application
PDP1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western Blot at 1:2000 dilution Lane 1: 293T/17 whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: L6 whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:2000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
