Application
Western blot analysis of lysate from WiDr cell line, using hPDX1R444 at 1:1000 at each lane.Application
Western blot analysis of lysate from WiDr cell line, using hPDX1R444 at 1:1000 at each lane.| Product Name | PDX1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Pyruvate dehydrogenase protein X component, mitochondrial, Dihydrolipoamide dehydrogenase-binding protein of pyruvate dehydrogenase complex, E3-binding protein, E3BP, Lipoyl-containing pyruvate dehydrogenase complex component X, proX, PDHX, PDX1 |
| Product description | PDX1, located in the mitochondrial matrix, is required for anchoring dihydrolipoamide dehydrogenase (E3) to the dihydrolipoamide transacetylase (E2) core of the pyruvate dehydrogenase complexes of eukaryotes. This specific binding is essential for a functional PDH complex. Eukaryotic pyruvate dehydrogenase complexes are organized about a core consisting of the oligomeric dihydrolipoamide acetyl-transferase, around which are arranged multiple copies of pyruvate dehydrogenase, dihydrolipoamide dehydrogenase and protein X bound by noncovalent bonds. Defects in PDHX are a cause of lacticacidemia. PDX1 belongs to the 2-oxoacid dehydrogenase family and contains 1 lipoyl-binding domain.1) Ling, M., et al., Hum. Mol. Genet. 7(3):501-505 (1998). Harris, R.A., et al., J. Biol. Chem. 272(32):19746-19751 (1997). Murray, J., et al., FEBS Lett. 529 (2-3), 173-178 (2002). |
| Immunogen | This PDX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 429-459 amino acids from the C-terminal region of human PDX1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | PDHX |
|---|---|
| Alternative Names | Pyruvate dehydrogenase protein X component mitochondrial Dihydrolipoamide dehydrogenase-binding protein of pyruvate dehydrogenase complex E3-binding protein E3BP Lipoyl-containing pyruvate dehydrogenase complex component X proX PDHX PDX1 |
| Molecular Weight(MW) | 54 kDa |
Application
Western blot analysis of lysate from WiDr cell line, using hPDX1R444 at 1:1000 at each lane.
Application
Western blot analysis of lysate from WiDr cell line, using hPDX1R444 at 1:1000 at each lane.| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
