Application
Immunofluorescence of PEAR1 in human kidney tissue with PEAR1 antibody at 20 μg/mL.Application
Immunohistochemistry of PEAR1 in human kidney tissue with PEAR1 antibody at 2.5 μg/mL.Application
Western blot analysis of PEAR1 in rat kidney tissue lysate with PEAR1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Product Name | PEAR1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PEAR1 Antibody: JEDI, MEGF12, Platelet endothelial aggregation receptor 1, Multiple epidermal growth factor-like domains protein 12, hPEAR1 |
| Product description | PEAR1 Antibody: Platelet endothelial aggregation receptor 1 (PEAR1) is a platelet receptor that signals upon the formation of platelet-platelet contacts independent of platelet activation and secondary to platelet aggregation. Upon platelet aggregation stimulated by physiological agonists, PEAR1 becomes tyrosine- and serine-phosphorylated; the tyrosine phosphorylation can be inhibited by eptifibatide, an aIIbbeta3 antagonist that also inhibits platelet aggregation. Recent studies have indicated that genetic variations in PEAR1, may be associated with enhanced agonist-induced platelet aggregation.1) Nanda N, Bao M, Lin H, et al. Platelet endothelial aggregation receptor 1 (PEAR1), a novel epidermal growth factor repeat-containing transmembrane receptor, participates in the platelet contact-induced activation. J. Biol. Chem.2005; 280:24680-9. |
| Immunogen | PEAR1 antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of human PEAR1. The immunogen is located within the last 50 amino acids of PEAR1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| PEAR1 antibody can be used for detection of PEAR1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PEAR1 |
|---|---|
| Alternative Names | PEAR1 Antibody: JEDI MEGF12 Platelet endothelial aggregation receptor 1 Multiple epidermal growth factor-like domains protein 12 hPEAR1 |
| Sequence Similarities | 2) Cat. No. 10-401 - Human Kidney Tissue Slide |
| Entrez Gene | 375033 |
|---|---|
| Protein Accession | NP_001073940 |
Application
Immunofluorescence of PEAR1 in human kidney tissue with PEAR1 antibody at 20 μg/mL.
Application
Immunohistochemistry of PEAR1 in human kidney tissue with PEAR1 antibody at 2.5 μg/mL.
Application
Western blot analysis of PEAR1 in rat kidney tissue lysate with PEAR1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1465 - Rat Kidney Tissue Lysate |
|---|---|
| Application Notes | PEAR1 antibody can be used for detection of PEAR1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | PEAR1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | PEAR1 Antibody is supplied in PBS containing 0.02% sodium azide. |
