PEG3 induces apoptosis in cooperation with SIAH1A and acts as a mediator between TP53/p53 and BAX in a neuronal death pathway that is activated by DNA damage. PEG3 acts synergistically with TRAF2 and inhibits TNF induced apoptosis through activation of NF-kappa-B. PEG3 also possesses a tumor suppressing activity in glioma cells.1) Dowdy, S.C., et al., (2005) Gynecol. Oncol. 99 (1), 126-134.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PEG3.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
PEG3 antibody can be used for detection of PEG3 by ELISA at 1:312500. PEG3 antibody can be used for detection of PEG3 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human A204 cells at 0.2-1 ug/ml.
Positive Control
1) A204 Cell Lysate
Application Notes
PEG3 antibody can be used for detection of PEG3 by ELISA at 1:312500. PEG3 antibody can be used for detection of PEG3 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store PEG3 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.