Application
Immunocytochemistry of Pellino in HepG2 cells with Pellino 1 antibody at 2.5 μg/ml.Application
Immunofluorescence of Pellino 1 in HepG2 cells with Pellino 1 antibody at 20 μg/ml.Application
Western blot analysis of Pellino 1 in human liver tissue lysate with Pellino 1 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | Pellino 1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Pellino 1 Antibody: PRISM, E3 ubiquitin-protein ligase pellino homolog 1, Pellino-related intracellular-signaling molecule, Pellino-1 |
| Product description | Pellino 1 Antibody: The Pellino proteins are a highly homologous family of E3 ubiquitin ligases that act as upstream mediators in Toll-like receptor (TLR) pathways that lead to activation of MAP kinases and transcription factors. Pellino 1 is required for interleukin-1-mediated signaling through its interaction with the IRAK4-IRAK-TRAF6 complex, ultimately resulting in the activation of NF-κB. Like other members of the Pellino family, Pellino 1 is an E3 ubiquitin ligase, able to catalyze the polyubiquitination of IRAK1. It is activated via phosphorylation by either IRAK1 and IRAK4 or the IKK-related kinases IKK-ε and TBK1. In addition to phosphorylation, Pellino 1 activity is also modulated via ubiquitination and sumoylation.1) Moynagh PN. The Pellino family: IRAK E3 ligases with emerging roles in innate immune signaling. Trends Immunol. 2009; 30:33-42. |
| Immunogen | Rabbit polyclonal Pellino 1 antibody was raised against a 15 amino acid peptide near the carboxy terminus of human Pellino 1. The immunogen is located within amino acids 300 - 350 of Pellino 1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| Pellino 1 antibody can be used for detection of Pellino 1 by Western blot at 1 - 2 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PELI1 |
|---|---|
| Alternative Names | Pellino 1 Antibody: PRISM E3 ubiquitin-protein ligase pellino homolog 1 Pellino-related intracellular-signaling molecule Pellino-1 |
| Molecular Weight(MW) | 46 kDa |
| Sequence Similarities | 2) Cat. No. 17-011 - Hep G2 Cell Slide |
Application
Immunocytochemistry of Pellino in HepG2 cells with Pellino 1 antibody at 2.5 μg/ml.
Application
Immunofluorescence of Pellino 1 in HepG2 cells with Pellino 1 antibody at 20 μg/ml.
Application
Western blot analysis of Pellino 1 in human liver tissue lysate with Pellino 1 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1304 - Human Liver Tissue Lysate |
|---|---|
| Application Notes | Pellino 1 antibody can be used for detection of Pellino 1 by Western blot at 1 - 2 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Pellino 1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Pellino 1 Antibody is supplied in PBS containing 0.02% sodium azide. |
