WB
Western blot analysis of PELI1 using anti-PELI1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PELI1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of PELI1 using anti-PELI1 antibody. PELI1 was detected in a paraffin-embedded section of human skin tissue. The tissue section was incubated with rabbit anti-PELI1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of PELI1 using anti-PELI1 antibody. PELI1 was detected in a paraffin-embedded section of rat lung tissue. The tissue section was incubated with rabbit anti-PELI1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of PELI1 using anti-PELI1 antibody. PELI1 was detected in a paraffin-embedded section of mouse lung tissue. The tissue section was incubated with rabbit anti-PELI1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.FC
Flow Cytometry analysis of PC-12 cells using anti-PELI1 antibody. Overlay histogram showing PC-12 cells stained with Anti-PELI1 Antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PELI1 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.IP
IP analysis of PELI1 using anti-PELI1 antibody in Caco-2 whole cell lysate. Western blot analysis of PELI1 using anti- PELI1 antibody. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti- PELI1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a mouse anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.| Product Name | Rabbit polyclonal antibody to PELI1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human PELI1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCIHCIPWB |
| WB:1:500-1:2000 IHC:1:50-1:400 FC:1:50-1:200 IP:1:250-1:300 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PELI1 |
|---|---|
| Gene Full Name | PELI1 |
| Gene Summary | Enables ubiquitin-ubiquitin ligase activity. Involved in several processes, including negative regulation of TORC1 signaling; negative regulation of necroptotic process; and protein polyubiquitination. Located in nucleolus and nucleoplasm. Is active in site of double-strand break. Implicated in colorectal cancer. [provided by Alliance of Genome Resources, Jun 2026] |
| Alternative Names | PELI1 Pellino 1 pellino homolog 1 (Drosophila) PRISM Protein pellino homolog 1 |
| Molecular Weight(MW) | 46 kDa(Observed band size: 40 kDa) |
| Cellular Localization | Chromosome. |
WB
Western blot analysis of PELI1 using anti-PELI1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PELI1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of PELI1 using anti-PELI1 antibody. PELI1 was detected in a paraffin-embedded section of human skin tissue. The tissue section was incubated with rabbit anti-PELI1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of PELI1 using anti-PELI1 antibody. PELI1 was detected in a paraffin-embedded section of rat lung tissue. The tissue section was incubated with rabbit anti-PELI1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of PELI1 using anti-PELI1 antibody. PELI1 was detected in a paraffin-embedded section of mouse lung tissue. The tissue section was incubated with rabbit anti-PELI1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
FC
Flow Cytometry analysis of PC-12 cells using anti-PELI1 antibody. Overlay histogram showing PC-12 cells stained with Anti-PELI1 Antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PELI1 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
IP
IP analysis of PELI1 using anti-PELI1 antibody in Caco-2 whole cell lysate. Western blot analysis of PELI1 using anti- PELI1 antibody. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti- PELI1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a mouse anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 FC:1:50-1:200 IP:1:250-1:300 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
