Application
Immunofluorescence of PEN2 in Rat Lung cells with PEN2 antibody at 20 μg/mL.Application
Immunohistochemistry of PEN2 in rat lung tissue with PEN2 antibody at 2.5 μg/mL.Application
Western blot analysis of PEN2 in A-20 cell lysate with PEN2 antibody at (A) 0.5 and (B) 1 μg/mL.| Product Name | PEN2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PEN2 Antibody: PEN2, PEN-2, MDS033, MSTP064, PEN2, Gamma-secretase subunit PEN-2, Presenilin enhancer protein 2 |
| Product description | PEN2 Antibody: PEN2, in addition to presenilin, nicastrin, and APH-1 forms the gamma-secretase protein complex, a membrane-bound aspartyl protease that can cleave certain proteins at peptide bonds buried within the hydrophobic environment of the lipid bilayer. This cleavage is responsible for a key step in signaling from several cell-surface receptors and is thought to be required for the generation of the neurotoxic amyloid peptides that are central to the pathogenesis of Alzheimer's disease. Like the tumor necrosis factor-alpha-converting enzyme (TACE) and the beta-site cleavage enzyme (BACE) protease families, gamma-secretase will cleave the amyloid precursor protein (APP), but within the intramembrane region of APP, resulting in either the non-toxic p3 (from the alpha and gamma cleavage site) or the toxic Abeta amyloid peptide (from the beta and gamma cleavage site). It is thought that accumulation of the Abeta peptide is the precursor to Alzheimer's disease.1) Weihofen A and Martoglio B. Intramembrane-cleaving proteases: controlled liberation of proteins and bioactive peptides. Trends Cell Biol. 2003; 13:71-8. |
| Immunogen | PEN2 antibody was raised against a 13 amino acid synthetic peptide from near the amino terminus of human PEN2. The immunogen is located within the first 50 amino acids of PEN2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| PEN2 antibody can be used for detection of PEN2 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PSENEN |
|---|---|
| Alternative Names | PEN2 Antibody: PEN2 PEN-2 MDS033 MSTP064 PEN2 Gamma-secretase subunit PEN-2 Presenilin enhancer protein 2 |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine: (100%) |
Application
Immunofluorescence of PEN2 in Rat Lung cells with PEN2 antibody at 20 μg/mL.
Application
Immunohistochemistry of PEN2 in rat lung tissue with PEN2 antibody at 2.5 μg/mL.
Application
Western blot analysis of PEN2 in A-20 cell lysate with PEN2 antibody at (A) 0.5 and (B) 1 μg/mL.| Positive Control | 1) Cat. No. 1288 - A20 Cell Lysate |
|---|---|
| Application Notes | PEN2 antibody can be used for detection of PEN2 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | PEN2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | PEN2 Antibody is supplied in PBS containing 0.02% sodium azide. |
