Application
Immunofluorescence of Periphilin in human colon tissue with Periphilin antibody at 5 μg/mL.Application
Immunohistochemistry of Periphilin in human colon tissue with Periphilin antibody at 2.5 μg/mL.Application
Western blot analysis of Periphilin in mouse colon tissue lysate with Periphilin antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Product Name | Periphilin Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Periphilin Antibody: HSPC206, HSPC232, Periphilin-1, Gastric cancer antigen Ga50 |
| Product description | Periphilin Antibody: Periphilin, known as PPHLN1 or gastric cancer antigen Ga50, plays a role in epithelial differentiation and contributes to epidermal integrity and barrier formation. It interacts with periplakin, a known precursor of the cornified cell envelope. Periphilin 1 is ubiquitously expressed and localizes to nucleus and cytoplasm. Existing as eight alternatively spliced isoforms, Periphilin is highly insoluble and contains a putative nuclear localization signal (NLS) within its N-terminal half, a prerequisite for the formation of insoluble complexes and a possible caspase recognition sequence and a potential nuclear export signal. Periphilin may play an important role in the nervous system.1) Kazerounian S and Aho S. Characterization of periphilin, a widespread, highly insoluble nuclear protein and potential constituent of the keratinocyte cornified envelope. J. Biol. Chem. 2003; 278:36707-17. |
| Immunogen | Periphilin antibody was raised against a 16 amino acid synthetic peptide near the center of human Periphilin. The immunogen is located within amino acids 160 - 210 of Periphilin. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| Periphilin antibody can be used for detection of Periphilin by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 5 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PPHLN1 |
|---|---|
| Alternative Names | Periphilin Antibody: HSPC206 HSPC232 Periphilin-1 Gastric cancer antigen Ga50 |
Application
Immunofluorescence of Periphilin in human colon tissue with Periphilin antibody at 5 μg/mL.
Application
Immunohistochemistry of Periphilin in human colon tissue with Periphilin antibody at 2.5 μg/mL.
Application
Western blot analysis of Periphilin in mouse colon tissue lysate with Periphilin antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1411 - Mouse Colon Tissue Lysate |
|---|---|
| Application Notes | Periphilin antibody can be used for detection of Periphilin by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Periphilin antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Periphilin Antibody is supplied in PBS containing 0.02% sodium azide. |
