Application
Immunocytochemistry of PERP in A431 cells with PERP antibody at 10 μg/mL.| Product Name | PERP Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PERP Antibody: THW, KCP1, PIGPC1, KRTCAP1, 1110017A08Rik, Krtcap1, p53 apoptosis effector related to PMP-22, Keratinocyte-associated protein 1, KCP-1 |
| Product description | PERP Antibody: The p53 tumor-suppressor gene integrates numerous signals that control cell life and death. Several novel molecules involved in p53 network, including Chk2, p53R2, p53AIP1, Noxa, PIDD, PID/MTA2, MTBP and PERP, were identified and their genes were cloned recently. PERP, also termed PIGPC1 and THW, is a plasma membrane protein. p53 binds to the promoter of PERP and transcriptionally activates PERP gene then the translated PERP protein mediates the p53 induced apoptosis. The expression of PERP causes cell death. PERP is a mediator of p53 induced apoptosis. PERP has sequence similarity to PMP-22/gas3 and is a new member of the PMP-22/gas3 family.1) Matsuoka S, Huang M, and Elledge SJ. Linkage of ATM to cell cycle regulation by the Chk2 protein kinase. Science 1998; 282:1893-7. |
| Immunogen | PERP antibody was raised against a synthetic peptide corresponding to 17 amino acids near the carboxy terminus of human PERP. The immunogen is located within the last 50 amino acids of PERP. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICCWB |
| PERP antibody can be used for detection of PERP by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Perp |
|---|---|
| Alternative Names | PERP Antibody: THW KCP1 PIGPC1 KRTCAP1 1110017A08Rik Krtcap1 p53 apoptosis effector related to PMP-22 Keratinocyte-associated protein 1 KCP-1 |
| Molecular Weight(MW) | Predicted: 27 kDa Observed: 30 kDa |
| Sequence Similarities | 2) Cat. No. 17-002 - A-431 Cell Slide |
Application
Immunocytochemistry of PERP in A431 cells with PERP antibody at 10 μg/mL.| Positive Control | 1) Cat. No. 1202 - A431 Cell Lysate |
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| Application Notes | PERP antibody can be used for detection of PERP by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL.: |
| Form | Liquid |
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| Storage Instructions | PERP antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | PERP Antibody is supplied in PBS containing 0.02% sodium azide. |
