Application
To detect hPersephin by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human Persephin (38-120) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hPersephin.| Product Name | Persephin Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PSPPersephinPSP |
| Product description | Persephin signals through a multicomponent receptor system, composed of RET and one of four GFR alpha (alpha1-alpha4) receptors. The GFRalpha4 was first identified in chicken and was later shown to be the preferential binding subunit for Persephin. In contrast to GDNF and Neurturin, Persephin does not support survival of peripheral neurons. |
| Immunogen | Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hPersephin. Human Persephin specific antibody was purified by affinity chromatography employing immobilized hPersephin matrix. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| ELISA: Indirect: To detect hPersephin by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hPersephin. Sandwich To detect hPersephin by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Human Persephin as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hPersephin. Western Blot: To detect hPersephin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPersephin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | PSPN |
|---|---|
| Alternative Names | PSPPersephinPSP |
Application
To detect hPersephin by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human Persephin (38-120) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hPersephin.| Application Notes | ELISA: Indirect: To detect hPersephin by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hPersephin. Sandwich To detect hPersephin by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Human Persephin as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hPersephin. Western Blot: To detect hPersephin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPersephin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Persephin antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
