Application
Flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).| Product Name | PEX14 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Peroxisomal membrane protein PEX14, PTS1 receptor-docking protein, Peroxin-14, Peroxisomal membrane anchor protein PEX14, PEX14 |
| Product description | This gene encodes an essential component of the peroxisomal import machinery. The protein is integrated into peroxisome membranes with its C-terminus exposed to the cytosol, and interacts with the cytosolic receptor for proteins containing a PTS1 peroxisomal targeting signal. The protein also functions as a transcriptional corepressor and interacts with a histone deacetylase. A mutation in this gene results in one form of Zellweger syndrome.1) Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010) Macgregor, S., et al. Hum. Mol. Genet. 19(13):2716-2724(2010) Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010)Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009) Shiozawa, K., et al. J. Biol. Chem. 284(37):25334-25342(2009) |
| Immunogen | This PEX14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 121-150 amino acids from the Central region of human PEX14. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PEX14 |
|---|---|
| Alternative Names | Peroxisomal membrane protein PEX14 PTS1 receptor-docking protein Peroxin-14 Peroxisomal membrane anchor protein PEX14 PEX14 |
| Molecular Weight(MW) | 41 kDa |
Application
Flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
