Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with PEX14 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Flow cytometry analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of PEX14 Antibody in HL-60 cell line lysates (35ug/lane)| Product Name | PEX14 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Peroxisomal membrane protein PEX14, PTS1 receptor-docking protein, Peroxin-14, Peroxisomal membrane anchor protein PEX14, PEX14 |
| Product description | PEX14 belongs to the peroxin 14 family. It is a component of the peroxisomal translocation machinery with PEX13 and PEX17 and interacts with both the PTS1 and PTS2 receptors. PEX14 binds directly to PEX17. Defects in PEX14 are the cause of peroxisome biogenesis disorder complementation group K (PBD-CGK) [MIM:601791] and Zellweger syndrome (ZWS) [MIM:214100].1) Neufeld C., et.al., EMBO J. 28:745-754(2009). |
| Immunogen | This PEX14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 343-370 amino acids from the C-terminal region of human PEX14. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PEX14 |
|---|---|
| Alternative Names | Peroxisomal membrane protein PEX14 PTS1 receptor-docking protein Peroxin-14 Peroxisomal membrane anchor protein PEX14 PEX14 |
| Molecular Weight(MW) | 41 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with PEX14 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Flow cytometry analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of PEX14 Antibody in HL-60 cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
