Application
Western blot analysis in NCI-H460 cell line lysates (35ug/lane).Application
PGAP3 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human colon tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Product Name | PGAP3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Post-GPI attachment to proteins factor 3, COS16 homolog, hCOS16, Gene coamplified with ERBB2 protein, PER1-like domain-containing protein 1, PGAP3, CAB2, PERLD1 |
| Product description | PGAP3 is involved in the lipid remodeling steps of GPI-anchor maturation. Lipid remodeling steps consist in the generation of 2 saturated fatty chains at the sn-2 position of GPI-anchors proteins. Required for phospholipase A2 activity that removes an acyl-chain at the sn-2 position of GPI-anchors during the remodeling of GPI (Probable).1) Bailey, S.D., et al. Diabetes Care (2010) In press : |
| Immunogen | This PGAP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 141-169 amino acids from the Central region of human PGAP3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PGAP3 |
|---|---|
| Alternative Names | Post-GPI attachment to proteins factor 3 COS16 homolog hCOS16 Gene coamplified with ERBB2 protein PER1-like domain-containing protein 1 PGAP3 CAB2 PERLD1 |
| Molecular Weight(MW) | 36 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Western blot analysis in NCI-H460 cell line lysates (35ug/lane).
Application
PGAP3 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human colon tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
