Application
PHB2 Antibody (Y248)immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PHB2 Antibody (Y248) for immunohistochemistry.Application
Western blot analysis of PHB2 using PHB2 Antibody (Y248) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PHB2 gene (Lane 2).Application
Western blot analysis of PHB2-Y248in MDA-MB435 cell line lysates (35ug/lane)Application
Western blot analysis of PHB2-Y248 in mouse kidney tissue lysates (35ug/lane)| Product Name | PHB2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Prohibitin-2, B-cell receptor-associated protein BAP37, D-prohibitin, Repressor of estrogen receptor activity, PHB2 {ECO:0000312|EMBL:AAH147661, ECO:0000312|HGNC:HGNC:30306} |
| Product description | PHB2 acts as a mediator of transcriptional repression by nuclear hormone receptors via recruitment of histone deacetylases. It functions as an estrogen receptor (ER)-selective coregulator that potentiates the inhibitory activities of antiestrogens and represses the activity of estrogens. It competes with NCOA1 for modulation of ER transcriptional activity and is probably involved in regulating mitochondrial respiration activity and in aging.1) Takata,H., Curr. Biol. 17 (15), 1356-1361 (2007) |
| Immunogen | This PHB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 225-255 amino acids from human PHB2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PHB2 |
|---|---|
| Alternative Names | Prohibitin-2 B-cell receptor-associated protein BAP37 D-prohibitin Repressor of estrogen receptor activity PHB2 {ECO:0000312|EMBL:AAH147661 ECO:0000312|HGNC:HGNC:30306} |
| Molecular Weight(MW) | 33 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
PHB2 Antibody (Y248)immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PHB2 Antibody (Y248) for immunohistochemistry.
Application
Western blot analysis of PHB2 using PHB2 Antibody (Y248) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PHB2 gene (Lane 2).
Application
Western blot analysis of PHB2-Y248in MDA-MB435 cell line lysates (35ug/lane)
Application
Western blot analysis of PHB2-Y248 in mouse kidney tissue lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
