Application
Western blot analysis of PHC3 in 3T3 cell lysate with PHC3 antibody at (A) 1 and (B) 2 μg/ml.| Product Name | PHC3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PHC3 Antibody: EDR3, HPH3, EDR3, PH3, Polyhomeotic-like protein 3, Early development regulatory protein 3, hPH3 |
| Product description | Polycomb group (PcG) proteins assemble into multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes throughout development (1). PHC3 (Polyhomeotic-like protein 3), also known as Early development regulatory protein 3, is a 983 amino acid nuclear protein that is a component of the PcG multiprotein PRC1 complex (2). PRC1 complex acts via chromatin remodeling and modification of histones. PHC3 contains 1 FCS-type zinc finger and SAM (sterile alpha motif) domain (2-3). PHC 3 has been regarded as a candidate tumor suppressor of osteosarcoma (4).1) Valk-Lingbeek ME, Bruggeman SW, and Van Lohuizen M. Stem cells and cancer; the polycomb connection. Cell 2004; 118:409–18. |
| Immunogen | PHC3 antibody was raised against a 19 amino acid peptide near the amino terminus of human PHC3. The immunogen is located within amino acids 240 - 290 of PHC3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| PHC3 antibody can be used for detection of PHC3 by Western blot at 1 - 2 μg/ml.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PHC3 |
|---|---|
| Alternative Names | PHC3 Antibody: EDR3 HPH3 EDR3 PH3 Polyhomeotic-like protein 3 Early development regulatory protein 3 hPH3 |
| Molecular Weight(MW) | Predicted: 109 kDa Observed: 120 kDa |
| Tissue Specificity | PHC3 antibody is human, mouse and rat specific. At least six isoforms of PHC3 are known to exist. |
Application
Western blot analysis of PHC3 in 3T3 cell lysate with PHC3 antibody at (A) 1 and (B) 2 μg/ml.| Positive Control | 1) Cat. No. 1282 - 3T3 (NIH) Cell Lysate |
|---|---|
| Application Notes | PHC3 antibody can be used for detection of PHC3 by Western blot at 1 - 2 μg/ml.: |
| Form | Liquid |
|---|---|
| Storage Instructions | PHC3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | PHC3 antibody is supplied in PBS containing 0.02% sodium azide. |
