Application
Immunofluorescence of PHF14 in 293 cells with PHF14 antibody at 5 μg/mL.Application
Western blot analysis of PHF14 in 293 cell lysate with PHF14 antibody at 1 μg/ml.| Product Name | PHF14 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PHF14 Antibody : KIAA0783, PHD finger protein 14 |
| Product description | PHF14, a novel plant homology domain (PHD) transcription factor, controls the proliferation of mesenchymal cells by regulating PDGFR alpha expression in embryonic development (1). PHD finger transcription factors are concerned with transcriptional regulation by interacting with modified histones (2), and dysregulation of several genes encoding PHD finger transcription factors, such as ING1 and RBP2, has been found in human cancer diseases suggesting these factors have an important role for cancer cell proliferation. PHF14 is a potential target for new treatments of lung fibrosis (1, 3).1) Kitagawa M, Takebe A, Ono Y, et al. Phf14, a novel regulator of mesenchyme growth via platelet-derived growth factor (PDGF) receptor-a. J. Biol. Chem. 2012; 287:27983-96. |
| Immunogen | PHF14 antibody was raised against a 19 amino acid peptide near the amino terminus of human PHF14. The immunogen is located within amino acids 210 - 260 of PHF14 . |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFWB |
| PHF14 antibody can be used for detection of PHF14 by Western blot at 1 - 2 μg/ml.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PHF14 |
|---|---|
| Alternative Names | PHF14 Antibody : KIAA0783 PHD finger protein 14 |
| Molecular Weight(MW) | Predicted: 98 kDa Observed: 100kDa |
| Sequence Similarities | 2) Cat. No. 17-010 - 293 Cell Slide |
| Tissue Specificity | PHF14 antibody is human, mouse and rat reactive. Multiple isoforms of PHF14 are known to exist. |
Application
Immunofluorescence of PHF14 in 293 cells with PHF14 antibody at 5 μg/mL.
Application
Western blot analysis of PHF14 in 293 cell lysate with PHF14 antibody at 1 μg/ml.| Positive Control | 1) Cat. No. 1210 - 293 Cell Lysate |
|---|---|
| Application Notes | PHF14 antibody can be used for detection of PHF14 by Western blot at 1 - 2 μg/ml.: |
| Form | Liquid |
|---|---|
| Storage Instructions | PHF14 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | PHF14 Antibody is supplied in PBS containing 0.02% sodium azide. |
