Application
Flow cytometric analysis of hela cells using PHGDH Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with PHGDH Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis of PHGDH using rabbit polyclonal PHGDH Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PHGDH gene (Lane 2).Application
Western blot analysis of PHGDH Antibody in mouse brain tissue lysates (35ug/lane)| Product Name | PHGDH Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | D-3-phosphoglycerate dehydrogenase, 3-PGDH, PHGDH, PGDH3 |
| Product description | 3-Phosphoglycerate dehydrogenase catalyzes the transition of 3-phosphoglycerate into 3-phosphohydroxypyruvate, which is the first and rate-limiting step in the phosphorylated pathway of serine biosynthesis, using NAD+/NADH as a cofactor.1) Klomp,L.W., et.al., Am. J. Hum. Genet. 67 (6), 1389-1399 (2000) |
| Immunogen | This PHGDH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 140-168 amino acids from the N-terminal region of human PHGDH. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Gene Symbol | PHGDH |
|---|---|
| Alternative Names | D-3-phosphoglycerate dehydrogenase 3-PGDH PHGDH PGDH3 |
| Molecular Weight(MW) | 57 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Flow cytometric analysis of hela cells using PHGDH Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with PHGDH Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis of PHGDH using rabbit polyclonal PHGDH Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PHGDH gene (Lane 2).
Application
Western blot analysis of PHGDH Antibody in mouse brain tissue lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
