Mutations in PHKG2 along with PHKA2 and PHKB, all three different genes of phosphorylase kinase (Phk) subunits, can give rise to glycogen storage disease of the liver. The autosomal-recessive, liver-specific variant of Phk deficiency is caused by mutations in the gene encoding the testis/liver isoform of the catalytic gamma subunit, PHKG2.1) Burwinkel, B., et al., (1998) Hum. Mol. Genet. 7 (1), 149-154.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PHKG2.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAIHCWB
PHKG2 antibody can be used for detection of PHKG2 by ELISA at 1:62500. PHKG2 antibody can be used for detection of PHKG2 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.
Application
Antibody used in IHC on Human kidney.
Positive Control
1) Cat. No. 1224 - Daudi Cell Lysate
Application Notes
PHKG2 antibody can be used for detection of PHKG2 by ELISA at 1:62500. PHKG2 antibody can be used for detection of PHKG2 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store PHKG2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.